Piscinibacter sakaiensis · seed A0A0K8P8E7 · 603 aa · family defined as ≥30% identity to that seed · compiled 07 October 2026
Open in CODSWALLOP UniProt A0A0K8P8E7 RCSB by accession PDBe-KB AlphaFold DB InterPro RCSB 6JTU PDBe
CATH and SCOP identifiers come from the RCSB's own structure annotations, which the Domains panel already reads, so these are looked up rather than guessed at.
Every figure here is counted over the whole family rather than quoted from one entry.
6JTU, the structure every other member of this family is superposed onto. Rendered by the RCSB and embedded here: the live app shows an interactive viewport, which a document that fetches nothing cannot.
How many of this family's constructs contain each residue of the seed. A trough is a stretch nobody has put in a construct, which is a construct-design answer rather than a disorder one.
6 distinct constructs across 10 entries. 10 polymer entities differ from the UniProt canonical sequence in some way, 4 carry a recognised expression tag and 0 carry a fusion partner.
"Differs from canonical" is not the same as "engineered". The canonical sequence is the full gene product, so a secreted protein whose structures all start after its signal peptide counts every one of them as different: lysozyme's most-used construct, residues 19–147 on 1,239 entities, is simply the mature protein. Read the construct column below for what was actually done, rather than this count.
| Entities | Length | Best (Å) | Best entry | What was made |
|---|---|---|---|---|
| 3 | 596 | 2.05 | 6QG9 | residues 8-603; L9N, L10H, A11K +8 more |
| 3 | 611 | 1.60 | 6QZ3 | His6 |
| 1 | 564 | 1.70 | 6QZ1 | residues 40-603 |
| 1 | 592 | 2.65 | 8EKG | residues 12-603; S12M, V13E, A14N +8 more |
| 1 | 613 | 2.10 | 6JTU | 1-residue insertion after 16; M1L, Q2A, T3V +10 more |
| 1 | 621 | 2.51 | 6JTT | His6; 1-residue insertion after 16; M1L, Q2A, T3V +10 more |
Columns where the wild-type residue still dominates but a real minority carries something else, which is a different question from "what varies across species".
| Oligomeric state | Chains | Entries | Share |
|---|---|---|---|
| monomeric | 1 | 10 | 100.0% |
2 entries have the depositor's assembly corroborated by PISA, 8 carry the depositor's word alone and 0 were assigned by PISA where the depositor gave none. The middle figure is not a disagreement: PISA may have returned nothing or never run.
| Component | Class | Name | Entries | Best (Å) |
|---|---|---|---|---|
| CA | ion | Calcium Ion | 10 | 1.60 |
| SO4 | ion | Sulfate Ion | 4 | 1.80 |
| EDO | cryoprotectant | 1,2-Ethanediol | 3 | 2.05 |
| BEZ | ligand | Benzoic Acid | 3 | 1.60 |
| ACT | cryoprotectant | Acetate Ion | 2 | 2.05 |
| MPD | cryoprotectant | (4s)-2-Methyl-2,4-Pentanediol | 2 | 2.10 |
| CL | ion | Chloride Ion | 2 | 2.10 |
| C9C | ligand | 4-(2-Hydroxyethyloxycarbonyl)benzoic Acid | 1 | 2.51 |
| C8X | ligand | Bis(2-Hydroxyethyl) Benzene-1,4-Dicarboxylate | 1 | 2.51 |
| GOL | cryoprotectant | Glycerol | 1 | 2.10 |
| FMT | buffer | Formic Acid | 1 | 2.10 |
| ZN | ion | Zinc Ion | 1 | 2.05 |
| J1K | ligand | 4-(2-Hydroxyethylcarbamoyl)benzoic Acid | 1 | 2.10 |
| PEG | cryoprotectant | Di(Hydroxyethyl)ether | 1 | 2.65 |
Parsed from the free text 10 depositors typed into
_exptl_crystal_grow.pdbx_details, out of 10
entries that recorded anything at all.
Median pH 6.5
(range 4.5 to 7.3).
10 entries carry a wwPDB validation report: 10 clean, 0 worth a check and 0 with something to explain. Median clashscore 3.79, median RSRZ outliers 1.25%, median R-free minus R-work 0.027. 10 have released structure factors.
| Organism | Entries | Best (Å) | Ligand-bound | Seed covered |
|---|---|---|---|---|
| Pseudideonella sakaiensis | 10 | 1.60 | 5 | 100% |
603 residues, numbered every ten. Every identity figure in this document is measured against this sequence.
active or binding site modified residue or glycosylation disulphide cysteine transmembrane or signal the 15 most-substituted positions
Sites are UniProt's curated features where the seed is a UniProt accession; the substituted positions are measured from this family's own alignment rather than annotated, and only the fifteen most substituted are marked: every position carrying a minority substitution would be most of the protein, because the family holds orthologues. A residue can carry more than one and is drawn with the first that applies, in the order of the key above.
One record per paper, not per entry.
| Year | Citation |
|---|---|
| 2024 | Increasing the Soluble Expression and Whole-Cell Activity of the Plastic-Degrading Enzyme MHETase through Consensus Design. Biochemistry doi:10.1021/acs.biochem.4c00165 |
| 2020 | Characterization and engineering of a two-enzyme system for plastics depolymerization. Proc.Natl.Acad.Sci.USA doi:10.1073/pnas.2006753117 |
| 2020 | Decomposition of PET film by MHETase using Exo-PETase function Acs Catalysis doi:10.1021/acscatal.9b05604 |
| 2019 | Structure of the plastic-degrading Ideonella sakaiensis MHETase bound to a substrate. Nat Commun doi:10.1038/s41467-019-09326-3 |
BEZ
C9C
C8X
J1K